Labs by Hers: The key markers that matter. Start testing

Nutrients

Vitamin D

Also known as: 25-hydroxyvitamin D test, 25(OH)D test, Calcidiol test, Vitamin D₂ and D₃ test

A vitamin D test measures how much 25-hydroxyvitamin D is in your blood — the main form of vitamin D circulating in your body. Vitamin D helps you absorb calcium and phosphorus, supporting strong bones, healthy teeth, and normal muscle and nerve function.

Providers may order this test if you have symptoms or risk factors for deficiency, such as fatigue, bone pain, or frequent illness. It’s also used to monitor conditions like osteoporosis, malabsorption, or kidney disease.

With telehealth, a provider can review your symptoms, order lab work, and interpret results. If your vitamin D level is too low or too high, a healthcare provider can determine whether it’s safe to manage remotely — for example, through supplements or diet changes — or if in-person care is needed.

Start testing
Learn more about Biomarkers

Key takeaways

  • Sample required: Blood (serum)

  • Tests for: Vitamin D status, which can help evaluate bone health, calcium balance, and certain metabolic or immune conditions

  • Healthy range: Typically 20–100 nanograms per milliliter (ng/mL).

What can a vitamin D test tell you?

A vitamin D test helps your provider:

  • Check bone and muscle health. Low levels can cause bone pain, muscle weakness, or fractures.

  • Evaluate calcium balance. Vitamin D works with calcium and parathyroid hormone to keep blood calcium steady.

  • Monitor chronic illness. It’s useful to know the vitamin D status of people with osteoporosis, kidney disease, or certain autoimmune conditions.

  • Track supplement response. Testing can reveal whether vitamin D therapy is improving levels.

What is being tested?

This test measures 25-hydroxyvitamin D, the storage form of vitamin D in the blood. The levels tested include:

  • Vitamin D₂ (ergocalciferol). From plant foods and supplements.

  • Vitamin D₃ (cholecalciferol). Made in the skin from sunlight and found in animal foods and supplements.

The liver converts vitamin D from sunlight or diet into 25-hydroxyvitamin D, which is then converted by the kidneys into 1,25-dihydroxyvitamin D (calcitriol) — the active form that helps regulate calcium and phosphate. Because 25-hydroxyvitamin D stays in the bloodstream longer, it’s the best indicator of vitamin D status.

Where is the vitamin D test typically included?

Most commonly, providers order vitamin D as an individual test to evaluate deficiency or monitor supplementation. The test measures total 25-hydroxyvitamin D (the sum of D₂ and D₃).

In some cases, it’s included alongside other labs — such as calcium, phosphorus, or parathyroid hormone — when assessing bone health or as part of diagnostic testing for kidney disease or metabolic disorders. It’s not automatically part of standard panels like the basic metabolic panel (BMP) or comprehensive metabolic panel (CMP).

Who should get a vitamin D test?

Testing may be recommended if you have symptoms or risk factors such as:

Symptoms:

Risk factors:

  • Limited sun exposure (northern climates, indoor lifestyle, sunscreen use)

  • Darker skin tone

  • Older age

  • Obesity

  • Digestive disorders (celiac disease, Crohn’s, ulcerative colitis)

  • Chronic kidney or liver disease

  • Long-term use of certain medications (glucocorticoids, anticonvulsants)

Providers also monitor vitamin D in people being treated for osteoporosis, osteomalacia, or parathyroid disorders, or anyone taking high-dose supplements.

How Labs by Hers works

  • 1

    Book your test

    Schedule a lab draw in the Hers app at one of more than 2,000 convenient locations.

  • 2

    Get your results

    Review your lab results to understand which areas of your health may need attention.

  • 3

    Spot early signals

    Identify potential health risks or system imbalances before they become more serious.

  • 4

    Get your action plan

    Receive expert guidance tailored to your health profile and test results.

  • 5

    Improve over time

    Track your progress through a mid-year panel and ongoing provider check-ins.

Not available in all 50 states. Eligibility and provider order required. Lab results alone are not intended to diagnose, treat, or cure any condition. A provider will reach out about critical results and plans include access to 24/7 provider messaging.

Images for illustrative purposes only.

Frequently asked questions

Start testing

What causes changes in vitamin D levels?

Vitamin D levels can fluctuate for several reasons related to sunlight exposure, diet, health conditions, and medication use. Low levels (deficiency) are often linked to limited time in the sun, low intake of vitamin D–rich foods, or conditions that reduce absorption, such as certain digestive disorders. Chronic kidney or liver disease, obesity, and medications like glucocorticoids, anticonvulsants, or some HIV treatments can also lower vitamin D. 

Conversely, high levels (excess) are less common but may result from taking too many supplements, consuming large amounts of fortified foods, or from rare medical conditions such as sarcoidosis or lymphoma, which can increase the body’s production of active vitamin D.

What is a normal range for vitamin D?

A healthy range for vitamin D is typically 20–100 nanograms per milliliter (ng/mL), though exact ranges vary by lab.

Can this test be done at home?

Yes. Some at-home kits use a small finger-prick sample mailed to a lab, though clinic tests are usually more precise.

What are the screening recommendations for vitamin D?

Testing is generally recommended for people at risk of deficiency — including those with limited sun exposure, darker skin tones, malabsorption disorders, or chronic kidney disease.

Do I need to fast before the test?

No fasting is required, but you may be asked to skip supplements the day before.

How long do results take?

Most labs report results within one to three business days.

How often should I get tested?

If your levels are normal and you don’t have risk factors, testing every few years is enough. People taking supplements or experiencing chronic illness may need checks every 6–12 months.

Does insurance cover it?

Usually, yes, when ordered for a medical reason such as bone disease or deficiency symptoms. However, insurance is not required for lab testing through Hers.

What happens if I don’t treat low vitamin D?

An untreated deficiency can weaken bones (osteomalacia or osteoporosis), raise fracture risk, and cause muscle weakness or fatigue.

Can I get enough vitamin D from sunlight alone?

It depends on location, skin tone, and time spent outdoors. Many people in northern climates or with darker skin require supplements or fortified foods.

What tests are related to this biomarker?

Calcium and phosphorus tests

• Parathyroid hormone (PTH) test

• Comprehensive metabolic panel (CMP)

• Bone density (DEXA) scan

Kidney and liver function tests

The information provided here is for informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always talk to a qualified healthcare provider about your specific health concerns.

Related biomarkers

Start testing

B12

Vitamin B12 supports red blood cell production, nerve health, and energy. Normal levels mean your body has enough supply to regulate your energy needs.

Learn more about B12

Ferritin

Ferritin is a protein that stores iron in the body, which is essential for oxygen transport. Normal levels mean your body has a healthy reserve to make red blood cells and transport oxygen, supporting energy and cognitive function.

Learn more about Ferritin

Ferritin/CRP ratio

This ratio compares ferritin, your iron storage protein, with C-reactive protein (CRP), a marker of inflammation. A normal ratio means there is a healthy balance between your iron stores and inflammation.

Learn more about Ferritin/CRP ratio

Folate, RBC

Folate, also known as vitamin B9, supports DNA production and red blood cell formation, and also helps break down homocysteine.

Learn more about Folate, RBC

Homocysteine

Homocysteine is an amino acid linked with folate, B12, and B6 metabolism. Healthy levels suggest your body is processing these vitamins effectively.

Learn more about Homocysteine

Iron

Iron helps make hemoglobin, which carries oxygen in the blood. Normal levels of iron support daily energy and focus.

Learn more about Iron

Iron Binding Capacity

This marker measures how well proteins in your blood (like transferrin) can carry iron. Normal levels mean your body is transporting iron effectively.

Learn more about Iron Binding Capacity

Iron % Saturation

Iron saturation measures how much iron is bound to transport proteins. Normal levels mean your tissues are getting the oxygen they need.

Learn more about Iron % Saturation

Magnesium, RBC

Magnesium supports muscle relaxation, heart rhythm, energy production, and bone health. Normal levels suggest your body has what it needs to regulate the muscles and nerves effectively.

Learn more about Magnesium, RBC

Zinc

Zinc supports immune defenses, wound healing, and sense of taste and smell. Healthy levels help to support your immune system.

Learn more about Zinc

Carbon Dioxide

Carbon dioxide helps your body maintain acid-base balance in the blood. Healthy levels protect energy and breathing.

Learn more about Carbon Dioxide

Calcium

Calcium supports strong bones, teeth, and steady muscle contractions. Calcium also plays a key role in heart rhythm and nerve signaling.

Learn more about Calcium

Chloride

Chloride helps your body maintain fluid balance, stomach acid production, and the right acidity (pH) for enzyme function (chemical reactions in the body).

Learn more about Chloride

Potassium

Potassium helps regulate heart rhythm, nerve signals, and muscle contractions. Eating fruits, vegetables, and legumes supports healthy levels.

Learn more about Potassium

Sodium

Sodium helps your body balance hydration, blood pressure, and nerve signaling effectively.

Learn more about Sodium

On your marks.

Get set.

Transform.

Find your baseline with 130+ biomarker tests, then plan for your health breakthrough.

Start testing

Supporting references

Sources

Reviewed by Lynn Marie Morski, MD

Published 12/04/2025